In vitro antioxidant activity of Calotropis gigantea hydroalcohlic leaves extract

By Namrata Singh, Neetesh K Jain, Pushpendra Kannojia, Navneet Garud, Anupam K Pathak and Swaroop C Mehta


Antioxidants play an important role to protect human against infections and degenerative diseases. The aim of this study is to verify the In-vitro antioxidant properties and to calculate the total polyphenol contents, total tannins, flavonoid contants of hydroalcohlic extract. The powdered crude drugs were extracted with hydro-alcoholic solvent (70:30) by double maceration process. Phytochemical tests of hydro-alcoholic extract reveals the presence of carbohydrate, alkaloid, flavonoid, steroids, protein, amino-acids and tannins. The antioxidant activity of hydroalcohlic extract of Calotropis gigantea leaves (HECGL) was investigated using in-vitro models like DPPH (1,1-Diphenyl-2-Picryl-Hydrazyl) free radical scavenging activity. Reducing power assay using ascorbic acid, nitric oxide scavenging activity using curcumin equivalents. At the same time the phenolic content of the extracts was determined using Folin-Ciocalteau reagent, total tannins and total flavonoids using rutine to evaluate their contribution to total antioxidant activity. HECGL at 400µg/ml concentration showed maximum DPPH radical scavenging activity (85.17%) and for nitric oxide scavenging (54.55%) at 100µg/ml concentration. Reducing power of HACGL was increases with increasing the concentration of extract. Phenolic contents, expressed as gallic acid equivalents 63.08 ± 4.17 /100 mg of the dried weight of cg. Tannin 0.52% and Flavonoids 46.97± 1.95 µg mg-1.It is concluded that, this study is to verify the antioxidant properties of hydroalcohlic extract, and to define the total polyphenol contents, flavonoids and tannins in Calotropis gigantea leaves.


Key Words : Antioxidant, Free radicals, Calotropis gigantea, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) , Nitric-Oxide.


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