RP-HPLC Method for Simultaneous Estimation of Thiocolchicoside  and Ketoprofen in Combined Dosage Form

By Sagar B.Wankhede, Somnath  S. Zambare, Nitin R.Dixit and Sohan S. Chitlange



Abstract

A RP-HPLC method has developed for simultaneous estimation of thiocolchicoside and ketoprofen in combined tablet dosage form. The mobile phase used was a combination of Acetonitrile: Water: Phosphate buffer (pH 3.0) (60:30:10, v/v/v). The detection of the combined dosage form was carried out at 260 nm and a flow rate employed was 1 ml/min. The retention time for thiocolchicoside and ketoprofen has found to be 2.70 and 4.90 min respectively. Linearity was obtained in the concentration range of 4-20 µg/ml of thiocolchicoside and 20-100 µg/ml of ketoprofen with a correlation coefficient of 0.9950 and 0.9997. The method was accurate and precise with recoveries in the range of 99.60 and 101.30 % for both the drugs and relative standard deviation (R.S.D.) < 2. The proposed method is highly sensitive, accurate and precise and hence was successfully applied for the reliable quantification of API content in the commercial formulation containing these drugs in combination. The developed method was validated according to International Conference on Harmonization guidelines.

 

Key Words : RP-HPLC, Thiocolchicoside,  Ketoprofen.

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