Simultaneous Determination of Mefenamic Acid and Ethamsylate by Area Under Curve Spectrophotometric Method

By Vinit D. Patil, Vinayak J. Kadam, Santosh N. Shinde, Aniruddha R. Chabukswar, Swati C. Jagdale, Bhanudas S. Kuchekar


Abstract

The method for the simultaneous determination of Mefenamic acid and Ethamsylate by spectroscopy has been developed. The simple, accurate and precise method includes Area Under the Curve (AUC) method. From a solvent effect studies and the spectral behaviours of Mefenamic acid and Ethamsylate, methanol was selected as solvent. Mefenamic acid shows maximum absorbance at 337 nm and Ethamsylate shows maximum absorbance at 306 nm. For the AUC method, the wavelength ranges between. 337-344 nm and 300-306 nm were selected with reference to the absorbance curves plotted between the wavelengths of 200-400 nm. This method allows rapid analysis of two drug combination. The results of analysis were validated statistically and by recovery studies by following ICH method validation guideline. Tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.

 

Key Words : Spectroscopy; Mefenamic acid; Ethamsylate; Area Under Curve Method.

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