By D. Boopathy, M. Prakash , Bibin Mathew, S. Suresh Kumar and P. Perumal
A simple, selective, rapid, and precise reverse phase HPLC method has been developed for the
simultaneous estimation of Ofloxacin and Satranidazole in pharmaceutical dosage form. A
Phenomenex Luna C18 (4.6*250 mm, 5µ) column was used for the separation. The mobile phase
was Phosphate buffer: Acetonitrile (70: 30 % v/v) and pH 6.0 at a flow rate of 1.2ml/min with
detection at 300nm.The retention time of Ofloxacin and Satranidazole was 3.720and 6.130min,
respectively. The developed method was validated in term of accuracy, precision, specificity,
system suitability, linearity, and robustness, limit of detection and limit of quantification. The
proposed method is suitable for simultaneous determination of Ofloxacin and Satranidazole in
pharmaceutical dosage form.
Key Words : Ofloxacin, Satranidazole, Simultaneous estimation and RP-HPLC.
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