By Pradeep Chauhan, Mahendra K. Panigrahi, Hemant Swami, Vivek Rana
Traditional techniques of callus and suspension culture do not fully explore the range possibilities of cultured cells. Immobilization is the advanced novel biotechnological approach, through which it is possible to achieve a physiological state conducive for metabolite production that may not have been attended otherwise. andrographolide is the major alkaloid found in Andrographis paniculata, has been used for the treatment of various diseases and disorders paerticulerly related with liver. Leaf cell homogenate was immobilized in calcium alginate and carragennan and the effect of immobilization on cell growth, cell viability and cells biosynthetic activity was observed.
Key Words : Andrographis paniculata, plant cell immobilization, secondary metabolite, andrographolide, HPLC.
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