Antifungal Activity of Embelia Ribes Plant Extracts
Sanjesh G Rathi , Vaidhun H Bhaskar , Bhuvan P. Raval, Maulik P. Suthar, Paras G Patel
Aim of the present study was to investigate the antifungal activity of the Embelia ribes plant extracts using standard in vitro antifungal susceptibility test methods like NCCLS (The National Committee for clinical laboratory standard M27-A2 Protocol).Antifungal screening of Embelia ribes not studied in detail and not extended to the different spectrum of fungal which are causing human diseases. Total four different types of extracts were prepared using different solvents and TLC characterized. Petroleum ether extract, Solvent ether extract, Methanol extract and water extract. Assays were performed in 96 well plates and detection was carried out with colorimetric plate reader at 530nm. With screening for the antifungal activity using NCCLS method, MIC50 were obtained with the help of the graph pad prism software. NCCLS method revealed that methanol extract and Embelin had lowest MIC50 range of 120mg/L against Candida albican (MTCCno.183) and among four Candida species tested Embelin had reported MIC50 values below 700mg/L. Solvent ether extract, petroleum ether extract, methanol extract and embelin reported to have MIC50 in range of 300-700mg/L against Candida albica (MTCCno.227)and Candida.parapsilosis(MTCCno.1744) Petroleum ether extract shows lowest MIC50 range of 250mg/L against Candida parapsilosis(MTCCno.1744)and 360mg/L against Candida laurintis (MTCC no.2898) while Water extract required higher MIC50 value for all species. Thus the result shows that the percentage growth was increased with the decrease in the concentration of the plant extracts, except for the water extract. The line for the water extract is roughly linear at all concentrations.
Key Words : Embelin, Embelia ribes, NCCLS method, TLC
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