Simultaneous estimation of plumbagin and embelin by reverse phase-high performance liquid chromatographic method 

By S. Gopinath, S. Muralidharan, S. Rajan, S. P. Danaphal 



A simple, rapid, precise RP-HPLC method was developed for simultaneous estimation of plumbagin and embelin containing different extract. The maximum resolution was achieved by using mobile phase acetonitrile: 50 mM potassium dihydrogen phosphate buffer: acetonitrile in the ratio (45:55) at pH 3.5.This mixture was found to be appropriate allowing good separation of both the components at a flow rate of 1 ml/min and detection wavelength 290 nm. Qualitative and quantitative results obtained using an HPLC method are described, which will be useful for developing highly effective herbal drug.

Key Words : Plumbago zeylanica; Embelia ribes; Extracts; HPLC/UV

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