In vitro Callus Culture of Adhatoda Vasica: A Medicinal Plant

 

By Sunita Maurya and  Dhananjay Singh 

ABSTRACT

Callus culture of Adhatoda vasica Nees were established from leaf explant. Different growth regulators greatly influenced the growth of callus cultures. Leaf derived callus grown on Murashige and Skooge (MS) medium  fortified with different concentration (1, 1.5, 2,2.5 ppm) of  Indole Acetic Acid (IAA), 6-Benzyladenine (BA), Kinetin (Kn),  2, 4- Dichlorophenoxyacetic Acid (2, 4-D). Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine and vasicinone in callus culture was detected and confirmed by thin layer chromatography. The Rf  values of vasicine and vasicinone were found as 0.52 and 0.60           

Keywords: Adhatoda vasica, Callus culture, Pyrroloquinazoline, Vasicine, Vasicinone 

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