Behavioral assessment studies in Cerebral ischemia induced by Bilateral carotid artery occlusion in rats 

By M. Nandagopal, P. Muralidharan, G. Thirumurugan 

Abstract

Ischemic stroke is the third leading cause of death and leading neurological cause of disability. The treatment of chronic behavioral loss following stroke is considered a major problem in the field of medicine. In our study effort has been made to develop new treatment ischemic stroke, ischemia was induced in rats by temporary bilateral carotid artery occlusion method (BCAO) in rats for 10 minutes followed by reperfusion. The animal behavior was assessed by neuromuscular function, Vestibulomotor function, Complex neuromotor function tests and social recognition by Juvenile recognition tests and upper extremity impairment by skilled reaching analysis test. Moreover, in-vivo tests such as Motor activity, Open field activity, Rotor rod and Morris water maze examinations were carried out to assess other behavior. Methanolic extract of Asparagus racemosus (MEAR) a well known rasayana herb was used for the treatment of ischemia induced animals. Treatment with MEAR showed considerable improvements in behavior of animals and significance of (P<0.01) done by ANOVA and Dunett’s in all behavioral assessments in a dose dependent manner. The results indicate the neuroprotective nature of MEAR and reduce the severity of stroke in rats and will enable them to rehabilitate affected body areas more quickly as normal rats. 

 

Key Words : Methanolic extract of Asparagus racemosus, Bilateral carotid artery occlusion, Behavior, Neusromuscular, water maze, Neuroprotection.

 

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