Spectrophotometric determination of zinc in blood serum of diabetic patients using bis-[2,6-(2´-hydroxy-4´-sulpho-1´-napthylazo)]pyridine disodium salt


By Banjit Barman, Sudarsan Barua



Bis-[2,6-(2´-hydroxy-4´-sulpho-1´-napthylazo)]pyridine disodium salt (HSNP), a new heterocyclic azo dye  is proposed as a  sensitive spectrophotometric reagent for zinc. A selective spectrophotometric method is presented for the trace determination of zinc in blood serum using HSNP as spectrophotometric reagent (?max = 565 nm) in basic aqueous solution (pH range = 7.5 to 9.1). The HSNP forms a 1:1 purple coloured complex. The Sandell’s Sensitivity is 1.37× 10-3 dm3 mol-1 cm-2 with molar absorptivity 4.6 ×104 mol L-1 cm-1. The proposed method has been successfully applied to the determination of zinc in blood samples of diabetic patients. The precision and the accuracy obtained were satisfactory.

Keywords:Bis-[2,6-(2´-hydroxy-4´-sulpho-1´-napthylazo)]pyridine disodium salt (HSNP), spectrophotometry, zinc,  blood serum, trichloro acetic acid (TCA).

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